special ecological reason for the presence of agarolytic bacteria in fresh water but that Identification of Medical Bacteria, 2nd edn. Cam-. Endolytic β-agarase Aga2 was identified from Cellulophaga omnivescoria W5C. SJP92 was shown to retain almost 90% of agarolytic activity under Recently, thermostable agarases from marine bacteria Flammeovirga sp. Abstract: Agarolytic bacteria use agarase to utilize agar as sole source of carbon. It is usually observed in life sciences labs that lot of agar medium needs to be.

Author: Yoramar Daishicage
Country: Russian Federation
Language: English (Spanish)
Genre: Technology
Published (Last): 23 February 2016
Pages: 299
PDF File Size: 11.41 Mb
ePub File Size: 19.21 Mb
ISBN: 578-6-20672-490-6
Downloads: 86473
Price: Free* [*Free Regsitration Required]
Uploader: Maumi

Some of the bacterial isolates have been assigned to the genera Alteromonas 12212733Cytophaga 43Streptomyces 36Vibrio 339and Pseudomonas Hydrolysis products of agar by agarase from P. Phylogenetic analysis of 16S rRNA. This possibility seems feasible because the enzyme could not be eluted from agarose columns as it is on other agarases 3. Van Hofsteen B, Malmqvist M.

Toffanin for the NMR technical support. Purification of agarase N In both cases the enzyme showed a molecular mass of 16 kDa, indicating an interaction with these resins. A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein-dye binding. Colonies that formed pits or clearing zones on agar were picked up and purified further by the same plating method.

There was a problem providing the content you requested

The molecular mass of the enzyme was estimated by gel filtration by using Sephadex G25 and Superdex 75 columns. We describe here the identification of a new agarolytic bacterial strain, P. The rDNA sequence of strain N-1 was compared to sequences available from public databases.


Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. The unrooted tree was constructed by neighbor-joining analysis.

Further studies on the characterization of new agarases and their coding genes will be required to determine the significance of these conserved regions. The enzyme gave a single band on SDS-polyacrylamide gels Fig. Isolation and characterization of Cytophaga flevensis sp.

Phylogenetic analysis and alignment. Sequence identificaion of the agaB gene encoding a new agarase from Vibrio sp.

Isolation And Identification of agarolytic bacteria in Marin by Faizah Rahman on Prezi

DNA base composition of Rickettsia tsutsugamushi determined by reversed-phase high-performance liquid chromatography. The enzyme was slowly released from the DEAE-cellulose by a washing with 1. Previous results on the purification and characterization of an extracellular agarase from the agar-liquefying strain Alteromonas sp. The amount of pigment was dependent on the addition of tyrosine to the culture medium, suggesting the presence of a melaninlike pigment 2.

Cloning and sequencing of agaAa unique agarase gene from a marine bacterium. In solid agar, this isolate produced a diffusible agarase that caused agar softening around the colonies.

Christen for carrying out the phylogenetic analysis and to R. The highest level off agarase was reached during the stationary phase. Guinea University of Barcelona, Barcelona, Spain The purified protein was determined to be homogeneous on the basis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it had agarolutic molecular mass of 33 kDa. The release of proteases into the medium during the stationary phase was demonstrated utilizing Azocoll Calbiochem-Behring, La Jolla, Calif.


Current protocols in molecular biology.

The amount of protein in the column fractions was determined by measuring the A When enzyme activity was measured in the presence of NaCl in concentrations of up to 0. We are grateful to R. The enzyme was incubated in 50 mM sodium phosphate at pH 6. Utilization of N -acetylglucosamine, cellobiose, d -fructose, d -galactose, d identifidation, glycogen, inulin, lactose, maltose, d -mannose, mannitol, sacarose, and d -xylose. Author information Article notes Copyright and License information Disclaimer.

Agwrolytic this step the enzyme eluted in the flowthrough, indicating that the strong binding seen at the beginning of the purification could be mediated by an unidentified extracellular component of this strain or by an agar-derived product that is separated during the gel filtration step.

No activity was observed when other carbon sources, such as glucose or galactose, were used instead of agar as the sole carbon source. At cruder stages the enzyme was strongly bound to DEAE-cellulose, probably through binding to a negatively charged agar or other polysaccharide. Nature London ;